ZFIN Image: Choe et al., 2013, Fig. S2

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Figure Caption

Fig. S2 Specificity of nkx2.3:Gal4VP16 and GFP-Dvl localization in pouches, related to Figure 3.

(A) Time-course of CFP-NTR expression in nkx2.3:Gal4VP16; UAS:CFP-NTR; her5:mCherryCAAX transgenic embryos. nkx2.3:Gal4VP16-dependent CFP-NTR expression (blue) co-localizes with her5:mCherryCAAX-positive pouch-forming endoderm (red) starting at 18 hpf, with expression becoming stronger as pouch development progresses. Views are dorsal-lateral with anterior to the left. Scale bar = 40 7mu;M. (B) Still images from a time-lapse confocal recording of fifth pouch development in nkx2.3:GFP-Dvl; her5:mCherryCAAX transgenic embryos (see Movie S5). GFP-Dvl (green) localizes to apical puncta (arrows) during remodeling of the her5:mCherryCAAX-positive endoderm (red) to form pouches. Scale bar = 20 μM.

Acknowledgments

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Reprinted from Developmental Cell, 24(3), Choe, C.P., Collazo, A., Trinh, L.A., Pan, L., Moens, C.B., and Crump, J.G., Wnt-Dependent Epithelial Transitions Drive Pharyngeal Pouch Formation, 296-309, Copyright (2013) with permission from Elsevier. Full text @ Dev. Cell