ZFIN Image: Kuo et al., 2013, Fig. 3

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Figure Caption

Fig. 3 Induction of ectopic chd and gsc expression by dnSox3 requires Fgf signalling.

Endogenous expression of chd and gsc (A,B) was inhibited by injection at 1–2 cell stage of a combination of morpholinos targeting both fgf3 and fgf8 (C,D), but these had little effect upon the ectopic expression of chd and gsc induced by injection of a dnSox3 construct (E,F). Treatment of embryos with the FGF signalling inhibitors, SU5402 (I,J) or MKP3 (O,P) (but not an equivalent concentration of the solvent DMSO alone) (G,H), at the 1–2 cell stage totally inhibited both endogenous expression of chd and gsc and ectopic expression induced by injection of a dnSox3 construct (K–N,Q,R). Lateral view and dorsal is to the right in upper panels, viewed from animal pole in lower panels. The proportion of embryos exhibiting these phenotypes is shown at the bottom right of each panel.

Acknowledgments

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