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Fig. S8 Sox3 overexpression is able to inhibit the ectopic expression of gsc induced by a range and combination of upstream factors. Embryos were injected with 50 pg of various RNAs (indicated above each panel) alone or combination and analysed for gsc (A) expression at 4.5 hpf. Injection of boz, fgf3, or boz combined with fgf3, caused expansion of gsc expression into the animal hemisphere of embryos (Ab–d). Injection of the additional up-stream factors, S37A (constitutive active β-catenin) and sqt strongly induced expansion of gsc throughout the entire animal hemisphere (Ae). However, co-injection with sox3 (B) reduced the expansion of expression of gsc expression or generated negative patches in the expansion of gsc expression no matter which other factors were injected. Although the combination of all factors still gave strongest ectopic expression, co-injection of sox3 was still able to generate gsc negative patches (Be). Lateral view and dorsal is to the right in upper panels, viewed from animal pole in lower panels. The proportion of embryos exhibiting these phenotypes is shown at the bottom right of each panel.