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Fig. S5 Single morpholinos targeting fgf3 or fgf8 have limited inhibitory effects on the expression of chd and gsc. Injection of an fgf3MO (5 ng) at the 1–2 cell stage caused a substantial, but incomplete, reduction in the domain of expression of chd and gsc at 4.5 hpf (A–D). Injection of an fgf8MO (5 ng) at the 1–2 cell stage caused a significant, but lesser, inhibition of chd and gsc expression (E,F). Lateral view and dorsal is to the right in upper panels, viewed from animal pole in lower panels. The proportion of embryos exhibiting these phenotypes is shown at the bottom right of each panel.
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