ZFIN Image: Chen et al., 2013, Fig. 2

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Figure Caption

Fig. 2 Transgenic neuron culture reveals presence of motor neurons and glial cells.

(A) Mn2Et motor neuron reporter line zebrafish at 48 hpf express GFP in spinal cord motor neurons that innervate axial trunk muscles, and at (B) 20 hpf express GFP in paired spinal cord motor neurons. (C) Cultured spinal cord neurons from mn2Et reporter line zebrafish express GFP in vitro. (D) Immunostaining for primary motor neuron antigen znp-1 and GFP confirms presence of motor neurons in mn2Et spinal cultures. (E) Immunostaining for GFP confirms presence of glial cells in Tg(olig2:EGFP) oligodendrocyte precursor reporter line spinal cultures. Scale bars, 50 μm (A) and 10 μm (B, C, D).

Acknowledgments

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