IMAGE

Fig. 5

ID

ZDB-IMAGE-130423-43

Genes

Antibodies

Ab1-tuba

Publication

Tay et al., 2013 - Lethal giant larvae 2 regulates development of the ciliated organ Kupffer's vesicle

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Figures for Tay et al., 2013

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Figure Caption

Fig. 5 Genetic interaction between Lgl2 and Rab11a regulates KV lumen formation uncoupled from ciliogenesis. (A,B) Images of GFP-labeled KV cells were used to determine KV lumen volumes (pseudocolored yellow) in live Tg(sox17:GFP) embryos (A) and acetylated tubulin antibodies were used to stain KV cilia (B) in MO control embryos and embryos injected with sub-optimal Rab11a MO^low, Lgl2 MO^low or co-injected with Rab11a MO^low + Lgl2 MO^low. The dashed circle outlines the approximate boundary of KV lumen. Scale bars: 20 μm. (C) Average volume of KV lumen at the 8-somite stage. (D,E) Analysis of KV cilia number (D) and length (E) showed that KV cilia were not significantly affected in Rab11a MO^low + Lgl2 MO^low embryos. Error bars indicate s.d. n, number of embryos analyzed. ^*P<0.05.

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Acknowledgments

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