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Fig. 1
Western blot analysis.
A. Representative Western blot for Opa1 yolk-less protein in MMC and Opa1 morphants. Opa1 protein is reduced at 24, 48, and 72 hpf. Differences were isoform specific. Samples from 24 hpf were imaged from a separate blot. Contrast was adjusted to improve visualization. *indicates isoforms selected for densitometry (see Figure 1B). Western blot results have been replicated with at least four independent injections B. Quantification of most intense Opa1 isoforms identified by Western blot. All values were first normalized to β-actin protein levels.
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