ZFIN Image: He et al., 2013, Fig. 3

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Figure Caption

Fig. 3

Transgenic zebrafish study.

(A) Schematic view of the Tol2 transposon-based plasmid carrying a 185 bp zebrafish nphs2 promoter. (B-D) Transient GFP expression in zebrafish podocytes at 4 dpf. Lateral view (B), confocal image (C), and dorsal view (D). Pronephros and dorsal aorta are indicated by arrows and an arrowhead, respectively. (E) GFP expression rate in 4 dpf-embryos injected with different constructs. The plasmid without inserts was used as a control for the baseline. The HCS3-A or -C sequence was subcloned upstream of the promoter. They are schematically shown in left panel. The bar graph illustrates expression rate (%) and total number of G₀ embryos for assessment are indicated in parentheses.

Acknowledgments

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