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Fig. 7
nkx2.5 and ltbp3 interact genetically during zebrafish SHF development. (A-C) nkx2.5 and ltbp3 morpholinos were injected alone or together into one-cell stage embryos. Total morpholino mass remained constant between experimental groups (see Materials and methods). Injected embryos were processed at 72 hpf for MF20 (myocardium; red) and Eln2 (OFT smooth muscle; green) double immunostaining. Embryos were binned into three phenotypic classes, unaffected (A), reduced (B) or absent (C), based on Eln2 OFT signal. White brackets highlight Eln2 staining. (D) Percentages of embryos in each phenotypic class according to experimental group. The n values are reported in the graph. OFT, outflow tract; V, ventricle; Ctrl, control; MO, morpholino.