ZFIN Image: Guner-Ataman et al., 2013, Fig. 3

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Figure Caption

Fig. 3

Ubiquitous reporter transgene corroborates specification of gata4⁺ and nkx2.5⁺ SHF progenitors in the ALPM. (A) Ubiquitous reporter transgene. (B-J) Hearts in 72 hpf double transgenic embryos treated transiently (75% epiboly to 14- to 16-somite stages) with ethanol [B-D; n=0/8 double transgenic embryos with cardiac mCherry reporter fluorescence; for nkx2.5 trace (hearts not shown), n=0/17 with reporter fluorescence] or 4-OHT (E-J) imaged in the green (B,E,H) and red (C,F,I) channels. Merged images are shown in D,G,J. n=18/18 double transgenic embryos with cardiac mCherry reporter fluorescence for E-G. n=15/15 with reporter fluorescence for H-J. OFT, outflow tract; V, ventricle; EtOH, ethanol; 4-OHT, 4-hydroxytamoxifen.

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