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Fig. 6 MZpak4 knockdown did not disturb early signaling pathways. (A and B) Convergent extension: double in situ hybridization with dlx3b and ntl probes was performed to outline the neural plate border and notochord at 3-somite stage. Dorsal view of anterior (A) and trunk (B) regions. The distance between dlx3b and the leading edge of ntl expression indicates extension (red line in A) while the width of ntl (red arrowheads in B) indicates convergence. The patterns in MZpak4 knockdown (bottom) are comparable to control embryos (top), indicating normal convergent extension in gastrulation. (C–F) Early markers for neural plate (sox2; C), hatching gland (hgg1; D) and neural crest (sox10; E and snai1b; F) were unaffected in pattern or intensity by MZpak4 knockdown (bottom embryos) compared to controls (top). Embryos were 12 somite stage in panel D, 3 somite stage in the other panels.
Reprinted from Mechanisms of Development, 130(2-3), Law, S.H., and Sargent, T.D., Maternal pak4 Expression is Required for Primitive Myelopoiesis in Zebrafish, 181-194, Copyright (2013) with permission from Elsevier. Full text @ Mech. Dev.