Fig. 6 More Texas Red fluorescence in hair cells when DDP-TR is solubilized in DMSO than in methanol.
Brn3c-GFP zebrafish were treated with 2 µg/mL DDP-TR dissolved in either methanol or 0.1% DMSO for two to forty-eight minutes and then fixed and imaged. (A) Low levels of DDP-TR fluorescence (red) were found in the O2 hair cells of methanol-treated zebrafish after two minutes. (B) More DDP-TR fluorescence was present in the hair cells of 0.1% DMSO-treated animals after two minutes. (C) DMSO increased levels of DDP-TR fluorescence into neuromast hair cells over time compared to DDP-TR plus methanol-treated fish. Results are the mean values ± SD. n = 15125 individual hair cells per time point per treatment. **p<0.01.