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Fig. 3 Loss of MLF axons in the hindbrain blocks FBMN migration. Maximum projection dorsal views of FBMNs (red) and the MLF (green) in 22 hpf Tg(zCREST1:membRFP) embryos stained using zn-12 antibody. (A) Uncut control embryos at 22 hpf show that FBMNs migrate in close proximity to the MLF. (B) Cuts made posterior to the hindbrain at the first somite (s1) do not affect FBMN migration. (C) Incomplete severing at r2 allows normal MLF axon and FBMN migration. (D) Severing of the embryo at r2 often results in FBMN migration into r6 on the LLF or projections of the reticulospinal (RS) neurons. (D′) zn-12 staining labels the LLF (white arrowheads), and the RS neurons (yellow arrowheads) and their projections (arrow). (E) Severing the MLF causes FBMN migration to stall in r5. (F) Percentage of embryos affected by manipulations. The number of embryos scored is indicated for each manipulation. Scale bar: 20 μm.