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Fig. 1 FBMNs migrate in close apposition to the MLF. (A-C) Maximum projection dorsal views of Tg(zCREST1:membRFP) embryos immunostained using zn-12 antibody show that FBMNs lie in close proximity to the MLF throughout their migration. zn-12 (green) labels the MLF (white arrowheads) and lateral longitudinal fasciculus (LLF, yellow arrowheads). (A) At 20 hpf, FBMNs (red) have migrated into r5 and the first FBMNs to migrate have reached r6 (arrows). (B) By 22 hpf, migration has continued with more FBMNs reaching r6. (C) At 24 hpf, FBMN migration into r6 continues. (D-F) Transverse sections of zCREST1:membRFP embryos at r5 stained using zn-12 show direct interaction between the MLF and FBMNs (white arrowheads) throughout FBMN migration at (D) 20 hpf, (E) 22 hpf and (F) 24 hpf. Asterisks highlight the otic vesicle. Broken lines indicate r5 boundaries for all figures. Scale bar: 20 μm.