ZFIN Image: Eisa-Beygi et al., 2013, Fig. 3

Image

Figure Caption

Fig. 3 Pharmacological inhibition of the HMGCR pathway results in loss of vascular stability and induces vessel rupture. ((A) and (B)) Representative bright-field micrographs of 48–52 hpf Tg(fli1:EGFP);(gata-1:DsRed) embryos treated with DMSO or 0.5 mg/L ATV. The boxed region shows where hemorrhage is observed. Scale bar=200 μm. ((C) and (D)) Representative composite confocal Z-stack projections of the boxed regions in the same Tg(fli1:EGFP);(gata-1:DsRed) embryos. Abbreviations: PrA, prosencephalic artery. Scale bar=40 μm. ((E) and (F)) Representative photomicrographs of 48–52 hpf embryos treated with DMSO or 0.5 mg/L ATV and subjected to fluorescence microangiography by intravascular injection of red-fluorescent microspheres. The white arrow shows the site of microsphere extravasation.

Figure Data

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 373(2), Eisa-Beygi, S., Hatch, G., Noble, S., Ekker, M., and Moon, T.W., The 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR) pathway regulates developmental cerebral-vascular stability via prenylation-dependent signalling pathway, 258-266, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.