Fig. 2

Fig. 2 Detection of postcranial NC derivatives. (A-D′) Lateral perspectives of epifluorescent acquisitions of DsRed⁺ components of the peripheral nervous system in induced Tg(sox10:ER^T2-Cre;β-actin:switch) metamorphic fish. (A) Sympathetic ganglia (white) located along the ventral walls of the dorsal aorta (the dorsal walls are highlighted by the dashed line) and axonal projections (arrowheads) towards the spinal chord. (B) Neurons located in the most posterior region of the gut (dashed lines), close to the anus, forming the enteric nervous system. (C) DRG with afferent (arrow) and efferent (arrowhead) projections. (D,D′) Somatosensory projections innervating the pectoral fin (D) and the caudal fin (D′); indicated are two of the most posterior DRG in the caudal region (arrowheads) and their projections entering the fin region (arrows). (E-F′) DsRed⁺ glia cells in induced Tg(sox10:ER^T2-Cre;β-actin:switch) metamorphic and adult fish. MIP of confocal stacks showing glia cells along the lateral line nerve (arrowhead) and the axonal projections innervating the neuromasts (arrows) in metamorphic (E) and adult (F) zebrafish. (F′) Lateral line glia in the caudal fin. (F,F′) Epifluorescence pictures. (G-I′) MIP of confocal stacks showing pigment cells in induced Tg(sox10:ER^T2-Cre;β-actin:switch) metamorphic fish: (G,G′) iridophore clone, (H,H′) melanophore clone with glia cells (arrowheads) and (I,I′) xanthophore clone with glia cells (arrowhead). (J) Confocal image of transverse cryosection and anti-DsRed antibody staining showing a labeled melanophore (arrow) close to labeled glia cells wrapped around the DRG. (K,K′) Heart region of induced metamorphic fish carrying the ubi:switch transgene as a reporter. (K) 3D view of confocal stacks showing cmlc:GFP⁺ cardiomyocytes (gray) and mCherry⁺ cells (red, arrow and arrowhead) in the heart. (K′) Confocal section of the region indicated in K by the arrow, showing colocalization between an mCherry⁺ cell and a GFP⁺ cardiomyocyte. (L) Confocal image of transverse cryosection and anti-DsRed antibody staining showing an induced Tg(sox10:ER^T2-Cre;β-actin:switch) metamorphic fish. Labeled adrenomedullary cells in the liver (red, inset). The gut is indicated by the asterisk. (M,M′) Confocal image of sagittal cryosection and anti-mCherry antibody staining showing the pronephric duct region of an induced Tg(sox10:ER^T2-Cre;ubi:switch) metamorphic fish. (M) mCherry⁺ cells populate the epithelial walls of the pronephric duct. (M′) The arrow indicates the otic capsule in the posterior chondrocranium.