Fig. S4 Microinjection of lmo2 1&2 morpholino (MO) in Tg(fli1:EGFP, gata1a: dsRed) zebrafish embryos. A-H - Morpholino (MO) mediated Lmo2 knockdown induces blood cells and blood vessel defects in 2 dpf Tg(fli1:EGFP, gata1a: dsRed) zebrafish embryos. A–D - non-injected control (NIC) and E – H 1.75 ng lmo2 1&2 MO injected zebrafish embryos. A–C, E – G Apotome microscope images (GFP/RFP merged) of lmo2 1&2 MO injected Tg(fli1:EGFP, gata1a: dsRed) embryos at 2 dpf (Lateral View). A–C - non-injected control embryos and E – G –lmo2 1&2 MO injected embryos. D, H - Embryos stained with o-dianisidine. The embryos were imaged at 10× magnification (A–C, E–G) and 2.5× magnification (D, H). Arrowheads indicate the site of defects.
Image
Figure Caption
Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and
ZFIN has permission only to display this image to its users.
Additional permissions should be obtained from the applicable author or publisher of the image.
Full text @ PLoS One