IMAGE

Fig. 5

ID
ZDB-IMAGE-130128-16
Source
Figures for Campbell et al., 2012
Image
Figure Caption

Fig. 5 Generation of a rod-specific, epitope-tagged, rtTA-expressing transgenic line for doxycycline-inducible gene expression.

(A) Diagram of the construct used to generate a stable transgenic zebrafish line that expresses a FLAG-tagged rtTA protein specifically in rod photoreceptors. The Xenopus rhodopsin promoter (Xla.rho) drives the flag epitope-tagged reverse tetracycline-controlled transcriptional activator (rtTAflag) gene. (B, C) Confocal z-projection of a retinal section from a 6 dpf Tg(Xla.rho:rtTAflag) larva labeled with anti-FLAG and anti-Rhodopsin antibodies. (B) Anti-FLAG immunofluorescence (red) is visible in the photoreceptor layer. (C) The anti-FLAG immunofluorescence (red) colocalizes with the anti-Rhodopsin immunofluorescence (blue) in the rod photoreceptors. dA, polyadenylation sequence; Tol2, pTol integration site. Scale bar, 50 µm.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One
Your Input Welcome
We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
Please check the highlighted fields and try again.
Thank you for submitting comments. Your input has been emailed to ZFIN curators who may contact you if additional information is required.
Oops. Something went wrong. Please try again later.