Fig. 2 Time course of doxycycline-induced GFP expression in Tg(Xla.rho:rtTA, TRE:GFP) larvae.
Confocal z-projections of retinal sections from 6 dpf Tg(Xla.rho:rtTA, TRE:GFP); alb/ larvae that were (A) untreated or treated with doxycycline (Dox) for (B) 1, (C) 2, (D) 4, (E) 8, (F) 16, (G) 24, (H) 48, or (I) 72 h. Anti-Rhodopsin immunofluorescence (red) labels rod photoreceptor outer segments and is visible for all treatment conditions. Anti-GFP immunofluorescence (green) is not visible in the untreated retinal section (A). The number of rods showing anti-GFP immunofluorescence (green) noticeably increases with an increase in the Dox exposure time (B–I). Scale bar (A), 50 µm.