Fig. S7

Fig. S7 Medial displacement of otpb:gfp-positive longitudinal axons is reduced in robo3 mutants after arnt2 knockdown. Dorsal views of z projections of the hindbrain of 72 hpf embryos labeled with anti-GFP and anti-3A10 antibodies are shown. Anterior is towards the left. (A,B) In twt^tw204/+;otpb:gfp embryos injected with arnt2 MO otpb:gfp-positive longitudinal axons (arrows in A) grow towards the midline. Midline crossing of Mauthner axons is normal (arrowhead in B). (C,D) After knock down of arnt2 in twt^tw204/tw204;otpb:gfp embryos, longitudinal axons project in a wildtype manner (arrows in C). Abnormal crossing of Mauthner axons (arrowhead in D) indicates homozygous robo3 mutant. (E) Quantification of the distance of MA neurons and of medio-lateral positioning of otpb:gfp-positive longitudinal axons at the anterior-posterior level of Mauthner neurons and medio-lateral positioning of otpb:gfp vagal axons. *P<0.001, Student′s t-test; n.s., not significant). Scale bar: 50 μm.