Fig. S5 Expression of robo3a.1 during development of hypothalamic DA neurons. Dorsal views of confocal z projections at the anterior-posterior level of the hypothalamus at indicated developmental stages. Anterior is towards the left. Only the left side of the brain is depicted. (A-C) At 24 hpf, expression of robo3a.1 is found in group 2 TH-positive DA neurons (see arrows in A,B). (D-F) At 48 hpf, expression of robo3a.1 in group 2 and group 4 TH-positive DA neurons is almost undetectable (arrowheads in D-E), whereas group 5-6 TH-positive DA neurons express robo3a.1. Numbers in B and E indicate DA neuronal groups according to Rink and Wullimann (Rink and Wullimann, 2002). Scale bars: 25 μm.
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