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Fig. S3 Loss of Sim1a function causes medial displacement of longitudinal otpb:gfp-positive axons. Dorsal views of hindbrain confocal z projections of otpb:gfp;hcrt:tdtomato double transgenic embryos at 72 hpf. (A-F) otpb:gfp axons (arrows in D,F) in sim1a morphants display strong medial displacement compared with controls (A,C) hcrt:tdtomato axons (arrowheads in B,C,E,F) are not affected. otpb:gfp hindbrain neurons (asterisks in A,D) do not contribute to longitudinal projections. (G) Quantification of mediolateral positioning of otpb:gfp and hcrt:tdtomato axons at the level of Mauthner neurons at 72 hpf after control or sim1a MO injection. ***P<0.0001; n.s., not significant. Scale bar: 50 μm for A-F.