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Fig. 6 Etv5b mediates Fgf signalling in the hypothalamus. Rescue experiment in which zebrafish eggs were injected with etv5b RNA and then treated with SU5402 (24-36 hpf). These embryos were compared with the effects of etv5b overexpression alone or SU5402 exposure alone. Embryos exposed to DMSO served as controls. (A-H) Confocal maximum intensity projections showing embryos subjected to the indicated treatments and processed for 5-HT immunohistochemistry. The intermediate/posterior (i./p.) clusters of the hypothalamus (A-D) and the 5-HT population of the anterior raphe (r.) are indicated (E-H). Ventral views, anterior left. Scale bar: 50 μm. (I) The number of 5-HT cells in the i./p. and r. clusters at 72 hpf after the indicated treatments expressed as percentage of control. n, total number of embryos analysed for each experiment. *Pd0.05; n.s., not significant.