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Fig. 1 TSA at 165 nM induces maximal acetylation of histone H3 and near-maximal acetylation of histone H4.
Immunoblot analysis of acetylated histones H3 and H4. WT zebrafish larvae at 48h.p.f. were incubated with TSA at various concentrations, DMSO, or no treatment, for 24hours. Lane 1 (8.25 nM TSA), 2 (16.5 nM TSA), 3 (41.25 nM TSA), 4 (82.5 nM TSA), 5 (165 nM TSA), 6 (330 nM TSA), 7 (825 nM TSA), 8 (0.5% DMSO), and 9 (no treatment). Total protein was extracted from each group of larvae at 72h.p.f., and analyzed by immunoblotting using the indicated antibodies. The intensity and area of each protein band was quantified by densitometric analysis. Each value represents the ratio of acetylated histone H3 (AcH3) to total histone H3, and acetylated histone H4 (AcH4) to H4, relative to that of control (no treatment).