ZFIN Image: Sakowski et al., 2012, Fig. 6

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Figure Caption

Fig. 6 MN loss in transgenic ALS zebrafish. Spinal cord cross sections from 30-60 week old zebrafish were stained with Cresyl violet Nissl (purple) to label MNs. (A) Representative low power images from control AB zebrafish is provided for orientation purposes; scale bar = 200 μm. Inset zoomed images of the spinal cord ventral horn from AB (A′′) and transgenic HB9:mGFP (A′) zebrafish validate our approach to quantify MN number based on cell body size and localization to the ventral horn; scale bar = 25 μm. (B-C) Representative images from control AB (B) and transgenic G93A-SOD1-GFP (C) zebrafish at 60 weeks of age. Arrowheads denote motor neurons; 20X magnification images; scale bar = 100 μm. (D) Quantification of MN counts per ventral horn in 30, 40 and 60 week old control AB and transgenic G93A-SOD1-GFP zebrafish. * P < 0.0001 compared to age-matched AB controls.

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Mol. Neurodegener.