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Fig. 3 Cell proliferation in regenerating young and old fish heart.
(A,B) Immunofluorescence images for Pcna (green) and Mef2 (magenta) of 14 dpa young (A) and old (B) fish hearts. Yellow arrowheads point to Pcna and Mef2 double positive, proliferating cardiomyocytes (shown as white signal). For simplicity, not all proliferating cardiomyocytes are pointed. Dotted lines indicate the amputated planes. Scale bar: 50μm. (C,D) Number of Pcna-positive proliferating cells per section (C), and Pcna-Mef2 double positive proliferating cardiomyocytes per section (D) of young and old fish hearts at 14 dpa. (E) Densities of cardiomyocytes in the regenerating area in 14 dpa hearts. The p-values between young and old fish are shown. Same slides were examined for C, D and E.
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