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Fig. 4 Whole-mount in situ hybridization of the myogenic regulatory factor myoD in cranial and trunk muscle precursors at 48 hpf.
Wild type siblings (a,a′); unc45a-/- (b,b′); unc45b-/- (c,c′); and unc45b-/-; unc45a-/- (d,d′) mutants. Compared to wild type siblings (a) myoD expression in the cranial muscle precursors is unchanged in the unc45a-/- (b), unc45b-/- (c), and unc45b-/-; unc45a-/- (d) mutants. unc45b-/- (c′) and unc45b-/-; unc45a-/- (d′) mutants have increased expression of myoD in the trunk precursors compared to wild type siblings (a′) and unc45a-/- mutants (b′). Primes denote alternative views of the same embryo. chv, constrictor hyoideus ventralis; im, intermandibularis; ir, inferior rectus; lr, lateral rectus; mr, medial rectus; pam, pharyngeal arch muscles; sh, sternohyoideus; so, superior oblique; sr, superior rectus.