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Figure Caption
Fig. 5 Efficacy of fgf5 splice-blocking MO.
(A) Schematic of fgf5 mRNA showing intron-exon structure (not to scale). Binding sites for splice-blocking morpholino at intron1-exon2 junction (I1E2-MO) and PCR primers for RT-PCR (forward P1, reverse P2) are shown. (B) RT-PCR results showing the efficacy of I1E2-MO. fgf5 transcript levels are severely reduced in fgf5 morphants at 24 hpf. odc transcript level was used as a constitutive control.
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