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Fig. S4

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ZDB-IMAGE-121217-16
Source
Figures for Nguyen-Chi et al., 2012
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Figure Caption

Fig. S4 Radar/gdf6a morpholinos-induced phenotype is reversed by WT rdr mRNA injection. (A-E) Live embryos at 1 dpf either (A) uninjected or (B) injected with rdr morpholinos (rdrMO) showing reduced eyes, massive cell death in the anterior neural tube and flatten somites (arrow heads, C1 phenotype). (C-D) Injections of 10–100 pg of rdr mRNA either alone (not shown) or in combination with rdrMO induce severe gastrulation defects as described in Sidi et al, 2003 (C2 and C3 phenotype). (E) Only a low quantity of rdr mRNA (0.5–1 pg) rescue the phenotype induced by rdrMO injection (C4 phenotype or WT). (F) Graphic representation of the percentage of embryos exhibiting WT, C1-C4 or dead phenotype in indicated conditions. (G-I) Prox1 (red) and Engrailed (green) expression in the somites of 1 dpf embryos either (G) uninjected or injected with (H) rdrMO alone or (I) in combination with 1 pg of rdr mRNA. Increased MP number observed in rdrMO injected embryos is reversed by WT rdr mRNA, showing that altered MP number is specifically induced by rdr loss-of-function.

Acknowledgments
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