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Fig. 6 BMP signaling forms a dorsal and ventral gradient within the myotome.
(A, B, C, D, E) Expression of GFP (green) and sMyHC (red) at indicated stages in Tg(5XBre[vent2]:-201id3:gfp) embryos uninjected or (E) after radar morpholino (rdrMO) injection. (F) Expression of phosphorylated-Smad5 (green) and nuclei (DAPI, blue) in 15-somite WT embryos. (G) Engrailed (red) and GFP (green) expression in Tg(5XBre[vent2]:-201id3:gfp) embryos at 20-somites. Cross-sections, maximum projections of multiple confocal scans. Scale bar 50 μm.
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