Fig. 6 Sae1 cell-autonomously regulates HSPC survival in the CHT. (A-D) Immunofluorescence staining of GFP in the CHT of 5 dpf host fish. (E-H) Immunofluorescence staining of Hbae1 in the CHT of 5 dpf host fish. (I-L) Merged images of GFP and Hbae1 immunofluorescence staining. A, E and I are the tangohkz5 sibling hosts transplanted with wild-type cells carrying CD41-eGFP. B, F and J are the tangohkz5/hkz5 mutants transplanted with wild-type cells carrying CD41-eGFP. C, G and K are the wild-type fish transplanted with tangohkz5 sibling cells carrying CD41-eGFP. D, H and L are the wild-type fish transplanted with tangohkz5/hkz5 mutant cells carrying CD41-eGFP. White dotted lines indicate the caudal vein.
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