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Figure Caption
Fig. 4 rb1 is required in RGC axons to regulate retinal exit.
(A-B) rb1 expression by RT-PCR from the 1-cell to 120 hpf larval stage and in the retina by in situ hybridization at 32 hpf. B-actin shown as control. White dashed circle marks lens position. (C) Schematic representation of retinal exit by ath5:gfp labeled transplanted RGC clones. (D-F) Transplanted ath5:gfp labeled clones from wild type donors to rb1te226 mutant retinas (D) and vice versa (E–F). White dashed oval marks retinal exit point. Arrow indicates intraretinally misrouted axons (E). Scale bar = 50 μm.
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