ZFIN Image: Lane et al., 2012, Fig. 6

Image

Figure Caption

Fig. 6 Elimination of mitfa and mitfb activity has no detectable effect on RPE development in zebrafish embryos.

mitfb cDNA showing exons, functional domains, and locations of mutations (arrows). The mitfb^hu3857 allele contains a T>A nonsense mutation that causes a switch from leucine to a stop codon in exon 2. The mitfb^hu3561 allele has a C>T nonsense mutation that results in a switch from Arginine to a stop codon in exon 7, in the basic-helix-loop-helix/leucine zipper binding domain (bHLH LZ). AD, transcriptional activation domain. (B) The mitfb^hu3857 allele creates an AlwNI restriction site, allowing genotyping of animals by electrophoresis of digested PCR products. (C–H) Bright field images depicting the normal RPE pigmentation and eye development in 5 dpf larvae; wildtype (C), or mitfb^hu3857 (D), mitfb^hu3561 (E), mitfa^w2 (F), mitfb^hu3857;mitfa^w2 (G), or mitfb^hu3561;mitfa^b692 (H) homozygous mutants.

Acknowledgments

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