ZFIN Image: Herbert et al., 2012, Fig. S2

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Figure Caption

Fig. S2 Knockdown of Hlx1 Using Gene-Targeted MO Reagents, Related to Figure 3
(A) Illustration of the hlx1 intron-exon structure indicating location of complementary PCR primers (arrows; F = forward, R= reverse) and MO-targeted sites. MO1 targets the first exon-intron boundary whereas MO2 targets the ATG start site.
(B) RT-PCR of hlx1 (using F and R PCR primers) or βactin1 using cDNA derived from embryos injected with either control MO or the indicated concentrations of MO1. MO1 dose-dependently alters hlx1 intron 1 splicing, resulting in a PCR product size shift.
(C-G) Lateral views of Tg(kdrl:GFP)^s843 embryos at 48 hpf (C-E) and quantification of the morphology of individual ISVs (F, G; half DLAV = an ISV connected to only one adjacent ISV; blunt ended ISV = an ISV with no connections to adjacent ISVs) upon injection of embryos with either control MO or the indicated concentration of MO1 (D; 12 ng) or MO2 (E; 4 ng). Hlx1 knockdown disrupts ISV sprouting. Throughout this study hlx1 MOs were used as a combination of 4ng MO1 and 1ng MO2.

Acknowledgments

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