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Fig. S7 CXCR4 antagonist treatment did not induce cell death. ( A-D ) Apoptotic cells detected using the TUNEL method (green, arrowhead) and CM marker Mef2 (magenta) were simultaneously detected. Control (A,C) and CXCR4 antagonist-treated heart (B,D) at 7 dpa (A,B) and 14 dpa (C,D) were analyzed. Shown are typical images from three hearts at each time point. Dotted lines indicate the amputation plane. TUNEL-positive CMs were barely detectable in both control and Cxcr4-inhibited heart. Scale bar: 50 μm.
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