ZFIN Image: Itou et al., 2012, Fig. S4

Image

Figure Caption

Fig. S4 FC131 effectively blocks directed migration of primordial germ cells in zebrafish. (A-D) In situ hybridization of vasa to visualize PGCs in control (A,C) and FC131-treated (B,D) zebrafish embryos at 18 hours post-fertilization. FC131 treatment was carried out between 8 and 18 hours post-fertilization. Lateral views (A,B) and ventral views (C,D) of typical samples from more than 50 embryos are shown. Black arrowheads point to PGCs localized at the region where the gonad develops, and white arrowheads point to mis-localized PGCs. Scale bar: 250 μm. (E) Dose-dependent activity of FC131. FC131 at 0, 4, 20, 40 and 400 nM were tested for PCG migration. Vertical axis represents percentage of embryos that exhibited abnormal PCG migration.

Acknowledgments

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