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Fig. S3 cxcl12a is expressed in the epicardial cells. (A,B) In situ hybridization for wt1b (A) and anti-DsRed2 staining (B) of adjacent sections from cxcl12a-DsRed2 reporter fish. (C,C′) A neighboring section, which is doubly stained for wt1b mRNA and anti-DsRed2. C′ shows higher magnification image of the boxed area in C. Arrowheads indicate signals where wt1b signals and Dsred2 signals overlap. Dotted line indicates the amputation plane. Shown are representative images from three independent experiments. (D-F) MHC staining (D), anti- DsRed2 staining (E) and merged image (F) from cxcl12a-DsRed2 reporter fish. Shown are representative images of multiple sections from two hearts. (G-I) fli1-EGFP reporter signal (G), cxcl12a-DsRed2 reporter signal (H) and merged image (I) from fli1-EGFP; cxcl12a-DsRed2 fish. Shown are representative images of multiple sections from two hearts. All images are from 3 dpa hearts. Scale bar: 50 μm.