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Fig. 5 Ginkgotoxin does not affect the development and appearance of zebrafish larvae. Zebrafish embryos were raised in fish water (control) or water containing 0.5 mM ginkgotoxin (GT) from 6 hpf to 72 hpf. (A–D) The lateral (A,C) and dorsal (B,D) views of embryos revealed no observable morphological differences in larval size and appearance between control and ginkgotoxin-treated groups. (E–H) The H&E-stained transverse cryosections of larval head regions and anterior trunk are oriented dorsal (top) to ventral (bottom) (E, F). Histochemical examination of the myotomes and notochord of embryos revealed no obvious difference between the control and ginkgotoxin-treated groups despite the variation in their shape (G,H). (I–P) The development of embryos were characterized at 24 hpf with in-situ hybridization using probes specific for somatic muscle (myo D, caudal lateral trunk views), rhombomere (krox20, lateral view with anterior to the left) and glutamatergic neurons (vglut2.1 and vglut2.2, dorsal view). (Q,R) The posterior lateral line formation was characterized at 72 hpf by staining with DASPEI. m, myotome; n, notochord; e, eyes; d, diencephalon; t, tectum; s, somite; r3, rhombomere 3; r5, rhombomere 5.