ZFIN Image: Stulberg et al., 2012, Fig. 4

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Figure Caption

Fig. 4 Quantification of Gsk3β, Akt and Erk phosphorylation in the tailbud following perturbation of Fgfr1, Mapk, PI3k and Gsk3β signaling. (A) Western blot analysis of pGsk3β after treatment with LiCl, SU5402, U0126 (Mapk inhibitor), and LY294002 (PI3k inhibitor), reveals levels are significantly down-regulated in LY294002 (n=11) and up-regulated in LiCl (n=9) treatments. (B) pAkt is significantly down-regulated by LY294002 (n=10) and (C) dpErk is down-regulated by U0126 (n=4), SU5402 (n=5), and is up-regulated by LiCl (n=5). Phospho-protein levels normalized to β-Tubulin levels. Statistical comparisons were made using the Student′s unpaired t test. *pd0.05, **pd0.01, ***pd0.001. Error bars are SEM.

Acknowledgments

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Reprinted from Developmental Biology, 369(2), Stulberg, M.J., Lin, A., Zhao, H., and Holley, S.A., Crosstalk between Fgf and Wnt signaling in the zebrafish tailbud, 298-307, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.