Fig. 2 SU5416 protects against MPTP-induced TH+ region area decrease in zebrafish.
One dpf zebrafish embryos were co-incubated with 200 μM MPTP and SU5416, VRI or 0.3% DMSO (vehicle control) at the indicated concentrations for 2 days. After treatment, zebrafish were collected to perform whole-mount immunohistochemistry. (A) Representative pictures of whole-mount immunostaining of zebrafish brain from different treatment groups. (B) Magnification of diencephalic area of zebrafish larval (indicated by red bracket in Fig. 2A). (C) Statistical analysis of TH+ region area in each treatment group (20 fish embryos per group). Data, expressed as percentage of control, were the mean ± SEM of three separate experiments; ##p<0.01 versus control; *p<0.05 and **p<0.01 versus MPTP group (Turkey’s test).