ZFIN Image: Sager et al., 2012, Fig. 6

Image

Figure Caption

Fig. 6 tor1 is expressed ubiquitously. A:

RNA in situ hybridization was employed to detect the tor1 transcript during development. Hybridized probe was detected using a histochemical reaction with a blue/purple product. The photomicrograph shows embryos at 12 hpf; the upper of the pair was hybridized with a tor1 antisense probe and the lower with a tor1 sense control probe. B: Reverse transcriptase PCR was employed to detect tor1_tv1 (upper panel) or tor1_tv2 (middle panel) mRNA in brain, foregut, hindgut, muscle, and gonads of adult zebrafish. Total RNA was treated with reverse transcriptase (RT⁺); controls lacking reverse transcriptase (RT^-) excluded amplification of genomic DNA sequences. bactin1 was amplified as a ubiquitously expressed control mRNA (lower panel).

Figure Data

Expression / Labeling details

Acknowledgments

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