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Fig. 3 Regenerative capability of zebrafish mandible.
The regenerated bone and cartilage are shown by Alcian blue staining. The first occurrence of the mandible regeneration was the chondrogenic differentiation indicated by cartilage blue-staining (10 dpa). At 21days after amputation, new bone formation began in an anterior direction and maintained the shape of the mandibular arch. In the following days, the cartilage blue-staining was reduced while red-skeletal stains increased. By two months the regenerated two ridges (mandibles) converged medially. Different from the uncut mandibular bones that were separated by a cartilaginous median symphysis, the regenerated mandibles appeared to fuse at the midline. Ms, mandibular symphysis; Ds, dorsal; V, ventral. Scale bars, 500 μm.