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Fig. S4
Expression of dorsoventral patterning genes in early embryos injected with low-dose mRNA for pou2-related genes. The expression of dorsoventral patterning genes, shown on the left, was examined at the shield stage in embryos injected with low-dose (30 pg/embryo) mRNA for egfp (A, F, K, P), pou2 (B, G, L, Q), vp-pou2 (C, H, M, R), mouse Oct-3/4 (D, I, N, S), and en-pou2 (E, J, O, T). Animal views with dorsal to the right (A?E, K?O) or lateral views with dorsal to the right and anterior to the top (F?J, P?T). The numbers of embryos with abnormal expression patterns and total numbers of embryos examined are shown at the bottom of the respective panels. The angles between the two dashed lines show the sizes of the gsc domain (A?E), and the arrows show the DV extent of the expression of chd (F?J) and eve1 (P?T). The expression of dorsal genes was reduced and ventral gene expression was expanded by pou2, vp-pou2, and mouse Oct-3/4. In contrast, en-pou2 overexpression gave rise to reverse effects. Scale bar, 200 μm.
Reprinted from Mechanisms of Development, 129(9-12), Khan, A., Nakamoto, A., Okamoto, S., Tai, M., Nakayama, Y., Kobayashi, K., Kawamura, A., Takeda, H., and Yamasu, K., Pou2, a class V POU-type transcription factor in zebrafish, regulates dorsoventral patterning and convergent extension movement at different blastula stages, 219-235, Copyright (2012) with permission from Elsevier. Full text @ Mech. Dev.