ZFIN Image: Khan et al., 2012, Fig. 11

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Figure Caption

Fig. 11

pou2 overexpression rescues the effects of HEP induction at 30% epiboly. Embryos from crosses of wild-type (WT) and HEP^+/- transgenic (Tg) fish were injected with egfp (second and third column) or pou2 mRNA (low dose, 30 pg/embryo; fourth and fifth column), exposed to heat shock at 30% epiboly, and allowed to develop until the bud stage at a lower temperature, at which time they were examined for marker expression. For the control, wild-type embryos (WT) were similarly stained (leftmost column). About half of the offspring embryos injected with egfp mRNA showed CE defects (third column). When pou2 mRNA was injected in advance, the ratio of embryos showing HEP-dependent effects was greatly decreased (rightmost column).

Acknowledgments

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Reprinted from Mechanisms of Development, 129(9-12), Khan, A., Nakamoto, A., Okamoto, S., Tai, M., Nakayama, Y., Kobayashi, K., Kawamura, A., Takeda, H., and Yamasu, K., Pou2, a class V POU-type transcription factor in zebrafish, regulates dorsoventral patterning and convergent extension movement at different blastula stages, 219-235, Copyright (2012) with permission from Elsevier. Full text @ Mech. Dev.