ZFIN Image: Khan et al., 2012, Fig. 7

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Figure Caption

Fig. 7

Effects of HEP induction at the sphere stage on the expression of dorsoventral patterning genes. Embryos from crosses of wild-type and HEP^+/- transgenic (Tg) fish were exposed to heat shock at the sphere stage for 1 h, allowed to develop at a lower temperature to 50% epiboly, and then examined for the expression of DV patterning genes (right two columns). Embryos in the third column showed normal patterns (Sibling-HS), whereas those in the rightmost column showed expanded (D, H, L, P) or reduced (T, X) expression (HEP-HS). The numbers of embryos with abnormal expression and total numbers of embryos examined are shown at the bottom of the respective panels in the HEP-HS column. For the control, gene expression was examined in parallel in wild-type embryos that were untreated (left column, Wild) or treated with heat shocks (second column, Wild-HS). Scale bar, 200 μm.

Acknowledgments

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Reprinted from Mechanisms of Development, 129(9-12), Khan, A., Nakamoto, A., Okamoto, S., Tai, M., Nakayama, Y., Kobayashi, K., Kawamura, A., Takeda, H., and Yamasu, K., Pou2, a class V POU-type transcription factor in zebrafish, regulates dorsoventral patterning and convergent extension movement at different blastula stages, 219-235, Copyright (2012) with permission from Elsevier. Full text @ Mech. Dev.