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Fig. S1 Expression analysis of Rest co-factors in MZrest mutants. (A,B) Expression levels of Rest co-factors assessed by qPCR at mid-blastula stage (4 hpf). Fold differences are relative to wild-type transcript levels (defined as 1). Expression levels of the three zebrafish sin3 homologs and rcor2 are comparable in wild-type and rest mutant embryos. rcor1 mRNA levels are enhanced by 0.75-fold in the rest mutant embryos. Error bars indicate s.e. from three pools of five embryos. (C-F) Whole-mount in situ hybridization (WISH) using rcor1 probe on wild-type and MZrest mutant embryos at 4 hpf (C,D) and 24 hpf (E,F).