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Fig. 6
Neutrophils Phagocytose and Kill Mycobacteria from Infected Granuloma Macrophages (A–C) Mean speed (A), sphericity (B), and bacterial volume (C) of infected neutrophils in 3 dpi granulomas, sampled from three granulomas each from different larvae. p value by Student′s unpaired t test (B) and by Student′s unpaired t test with Welch′s correction (A and C). (D) Representative frames of time-lapse laser confocal images of infected motile neutrophils in larva showing a neutrophil (white arrowhead) phagocytosing bacteria from a granuloma macrophage. (E) Bacterial burden over time and interval speed of neutrophil in (D). (F) Representative frames of time-lapse laser confocal images of infected motile neutrophil in larva showing neutrophil (white arrowhead) repeatedly phagocytosing bacteria (yellow and blue arrows) from a granuloma macrophage. (G) Bacterial burden over time and interval speed of neutrophil in (F) with yellow and blue arrowheads indicating respective phagocytic events in (F). In (D) and (F), green, neutrophil (N); red, Mm, minutes in upper right corner. Yellow objects (white arrowheads) showing bacteria within neutrophils are built by 3D surface rendering with Imaris. Also see Figure S4 and Movie S5, Movie S6, and Movie S7.