ZFIN Image: McGraw et al., 2012, Fig. 2

Image

Figure Caption

Fig. 2 Neural crest migration into the dorsal root ganglia (DRG) ceases prior to neuron differentiation. (A-A′′ ′) Confocal projections of stills from a time-lapse movie showing migrating Tg(sox10:nlsEos) + neural crest cells between 15 and 38 dpf. (A) At time zero, the region of DRG formation contains two Eos + cells. (A′) A still at 6:20 hours shows a representative neural crest cell joining the DRG anlagen (yellow arrowhead). Between 10:00 hours (A′′) and 23:10 hours (A′′ ′), no neural crest cells migrate into the DRG. (B-B′′ ′) Time-lapse movie a Tg(sox10:nlsEos)/Tg(neurod:TagRFP) transgenic embryo between 30 and 57:10 hpf. (B) An Eos+/RFP- cell (white arrowhead) at time zero, begins to express RFP by 0:40 hours (B′) and divides at 0:50 hours (B′′). (B′′ ′) After 26.30 hours of imaging, neurons are labeled with RFP (blue arrow). (C) Quantification of Eos + cells that migrate into the DRG anlagen between 18 and 48 hpf. No new neural crest cells join the DRG after 32 hpf (n = five embryos). Scale bars, 25 μm. dpf, days postfertilization; hpf, hours postfertilization.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Neural Dev.