IMAGE

Fig. 1

ID
ZDB-IMAGE-120905-10
Source
Figures for Harding et al., 2012
Image
Figure Caption

Fig. 1

Fgfr-Ras-MAPK signaling in the pLLp is required for rosette formation. (A-D) Confocal projections showing rosette formation in the DMSO control (A) and with Fgfr (B), Ras (C) and MAPKK (D) inhibition in claudinB:EGFP zebrafish embryos at 30 hpf. Arrowheads indicate centers of the trailing rosettes. Pink arrow indicates nascent rosette. Brackets indicate rosette-free region. (A2-D2) pMAPK immunolabeling in single planes from projections in A-D. Fgfr and MAPKK inhibition with 100 μM SU5402 and 7 μM PD0325901, respectively, was from 28-30 hpf. Ras inhibition via hsp70:dn-Ras induction was at 28 hpf; embryos were fixed 2 hours following heat shock. (E-G) Stills from time-lapse movie of pLLp in a wild-type claudinB:EGFP embryo (supplementary material Movie 1). (F) Cells align apical ends along the midline (pink arrows). (G) Center of the nascent rosette (arrowhead). (E2-G2) Three-dimensional reconstructions of the highlighted cell in E-G. (H) ACIs for embryos treated with DMSO, SU5402, PD0325901 or following induction of hsp70:dn-Ras. n=180 cells from six embryos per condition. **P<0.0001, Wilcoxon test. Error bars indicate s.e.m. Scale bars: 20 μm in A-G; 4 μm in E2-G2.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development