ZFIN Image: Delaurier et al., 2012, Fig. S5

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Figure Caption

Fig. S5

hdac4 mRNA splicing is down-regulated in MO-injected embryos. RT-PCR of cDNA from whole embryo RNA extractions at 24 hpf and 3 dpf. Combined injection of MO1 and MO2 resulted in down-regulation of normal splicing between exon-8 and exon-9 and exon-9 and exon-10 (e8e10 = PCR product showing splicing between exon-8 through exon-10 splicing; e9e11 = PCR product showing splicing between exon-9 through exon-11; e8i9 = PCR product showing mis-splicing resulting in inclusion of intron between exon-8 and intron-9; e9i10 = PCR product showing mis-splicing resulting in inclusion of intron between exon-9 and intron-10). The expression of mRNA with intronic sequence was higher in injected embryos than in uninjected controls. RT-PCR for β-actin (control) was performed to demonstrate total mRNA levels used for RT-PCR were equal between samples.

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Expression / Labeling details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.